全文获取类型
收费全文 | 17141篇 |
免费 | 1370篇 |
国内免费 | 896篇 |
出版年
2023年 | 161篇 |
2022年 | 159篇 |
2021年 | 604篇 |
2020年 | 460篇 |
2019年 | 570篇 |
2018年 | 717篇 |
2017年 | 515篇 |
2016年 | 779篇 |
2015年 | 1131篇 |
2014年 | 1281篇 |
2013年 | 1377篇 |
2012年 | 1654篇 |
2011年 | 1467篇 |
2010年 | 938篇 |
2009年 | 736篇 |
2008年 | 992篇 |
2007年 | 885篇 |
2006年 | 729篇 |
2005年 | 656篇 |
2004年 | 650篇 |
2003年 | 609篇 |
2002年 | 461篇 |
2001年 | 279篇 |
2000年 | 235篇 |
1999年 | 241篇 |
1998年 | 125篇 |
1997年 | 95篇 |
1996年 | 66篇 |
1995年 | 83篇 |
1994年 | 68篇 |
1993年 | 49篇 |
1992年 | 68篇 |
1991年 | 61篇 |
1990年 | 37篇 |
1989年 | 36篇 |
1988年 | 40篇 |
1987年 | 22篇 |
1986年 | 28篇 |
1985年 | 25篇 |
1984年 | 27篇 |
1983年 | 17篇 |
1982年 | 16篇 |
1981年 | 15篇 |
1979年 | 13篇 |
1975年 | 12篇 |
1974年 | 12篇 |
1973年 | 21篇 |
1972年 | 12篇 |
1966年 | 12篇 |
1958年 | 13篇 |
排序方式: 共有10000条查询结果,搜索用时 546 毫秒
81.
Jiří Fajkus Aleš Kovařík Robert mKrálovics Milan Bezděk 《Molecular genetics and genomics : MGG》1995,247(5):633-638
We have examined the structure and chromatin organization of telomeres in Nicotiana tabacum. In tobacco the blocks of simple telomeric repeats (TTTAGGG)n are many times larger than in other plants, e.g., Arabidopsis thatiana or tomato. They are resolved as multiple fragments 60–160 kb in size (in most cases 90–130 kb) on pulsed-field gel electrophoresis (PFGE) of restriction endonuclease-digested DNA. The major subtelomeric repeat of the HRS60 family forms large homogeneous blocks of a basic 180 by motif having comparable lengths. Micrococcal nuclease (MNase) cleaves tobacco telomeric chromatin into subunits with a short repeat length of 157±5 bp; the subtelomeric heterochromatin characterized by tandemly repeated sequences of the HRS60 family is cut by MNase with a 180 by periodicity. The monomeric and dimeric particles of telomeric and subtelomeric chromatin differ in sensitivity to MNase treatment: the telomeric particles are readily digested, producing ladders with a periodicity of 7 bp, while the subtelomeric particles appear to be rather resistant to intranucleosomal cleavage. The results presented show apparent similarities in the organization of telomeric chromatin in higher plants and mammals. 相似文献
82.
以IL-8免疫的BALB/C小鼠脾细胞与Sp2/0或653小鼠骨髓瘤细胞融合构建了淋巴细胞杂交瘤克隆I8-S2和I8-63。ELISA叠加试验(ELISA Additivity Test)表明这两杂交瘤克隆分泌的单抗分别识别IL-8分子的不同表位。IL-8能激活人颗粒细胞,引起细胞内Ca~(2 )浓度([Ca~(2 )]_i)上升。通过流式细胞仪分析[Ca~(2 )]_i的变化,发现两个克隆单抗对IL8激活细胞的活力具有不同的中和作用。克隆I8-S2具有很强的中和作用,而克隆18-63则不然。上述结果提示IL-8的激活细胞活力局限于该分子的某表位。 相似文献
83.
84.
Serological data identify a single major histocompatibility complex (MHC) class I locus in cattle. Molecular data, however, demonstrate the presence of at least two cattle MHC (BoLA) class I loci. To investigate the number of transcribed BoLA class I genes, we amplified cattle cDNA by using a single MHC class I-specific primer that hybridized to a conserved region of exon 4 and a non-specific 3 primer. Six BoLA class I cDNAs have been cloned and sequenced from a Bos taurus bull heterozygous for BoLA class I serological antigens, demonstrating the presence of a minimum of three loci. Sequence comparisons suggested that one of these cDNAs may be an unexpressed allele or the product of a nonclassical locus.The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession numbers U01186 and U01187. 相似文献
85.
G. Wang J. Ji Y-B. Wang H. Hu I. P. King J. W. Snape 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1993,87(5):531-536
Two novel 46-chromosome doubled haploid lines, W66 and M17, derived from separate hexaploid triticale x bread wheat crosses, were characterised using cytological and biochemical markers. Both lines were shown to be relatively stable cytologically, over 11 and 8 generations of selfing, respectively. By examining mitotic and meiotic chromosomes, the stabilities of the two lines were shown to be similar with frequencies of 2n=46 in 74.2–85.5% of cells. However, over selfed generations, the rye chromosomes were shown to have lost some of their heterochromatin, which made it difficult to establish their continued presence using cytological techniques, such as C-banding alone. Cytological evidence from pairing studies, C-banding, and fluorescence in-situ hybridization, showed that both M17 and W66 are wheat/rye multi-addition lines with rye chromosome constitutions of 1R+6R, and 1R+4R, respectively. These conclusions were confirmed by isozyme and storage-protein analysis. 相似文献
86.
Kyung Hoon Jung Jeong Hwan Kim Yeong Joong Jeon Jae Heung Lee 《Biotechnology letters》1993,15(1):65-70
Summary A novel two enzyme system of fructosyltransferase and glucose oxidase to enhance the content of the net fructo—oligosaccharide (FOS) fractions in the industrial production of FOS syrup from sucrose was devised. The net FOS content in the commercial FOS syrup has been limited only to 55–60 % due to the accumulation of glucose which acts as a feedback inhibitor of the fructosyltransferase. By supplementing glucose oxidase to the conventional FOS reaction system, we could convert the glucose to gluconic acid readily separable from neutral sugars by simple ion exchange operation in the next step. The simultaneous removal of glucose was proved effective in proceeding the reaction by fructosyltransferase further by relieving the product inhibition caused by glucose. By this way, we could raise the net FOS content as high as 90 %. 相似文献
87.
88.
89.
物种保护信息管理系统的开发应用 总被引:9,自引:1,他引:8
在英文物种、生境和保护区管理系(MASS 3.1)的基础上,研究并改编完成了其中文系统软件包(CMASS 2.0),并用此中文系统软件包建立了中国自然保护区信息库、中国保护动物信息库、中国濒危动物信息库、中国兽类信息库、中国鸟类信息库,这些库的建立对我国野生动物保护具有重要意义。 相似文献
90.
本文利用PCR技术,对人肿瘤坏死因子α(hTNFα)基因进行了改造,并将其与人白细胞介素-6(hIL-6)成熟肽编码区cDNA进行融合,构建了5′IL-6-TNF△融合蛋白的表达质粒pBVIL6-TNFA△。DNA序列分析证明,PCR扩增片段核苷酸序列与引物设计序列及相应的cDNA序列完全一致;重组子用限制性内切酶酶切鉴定,含有正确的IL6-TNF△融合cDNA片段;表达产物经SDS-聚丙烯酰胺凝胶电泳,分子量约为37kD,与预计的相符合;生物学活性分析初步表明,该融合蛋白具有抗肿瘤活性。 相似文献